Session profile
- Audience: learners needing robust neuron-glia boundary decisions.
- Duration: 70 minutes lecture + 20 minutes morphology drill.
- Output: glia recognition checklist and adjudication log.
Slide-by-slide lecture plan
- Slide 1 (2 min): Title and glia relevance
- Slide 2 (5 min): Why glia are not “background”
- Slide 3 (6 min): Class overview
- Astrocytes, microglia, oligodendrocytes.
- Slide 4 (6 min): Morphological and contextual cues
- Slide 5 (6 min): Vascular and myelin context interpretation
- Slide 6 (6 min): Glia-neuron ambiguity classes
- Slide 7 (7 min): Worked case I: astrocyte-like process
- Slide 8 (7 min): Worked case II: microglia confusion case
- Slide 9 (6 min): Worked case III: oligodendrocyte/myelin boundaries
- Slide 10 (5 min): QC metrics for glia labeling
- Slide 11 (5 min): Escalation and second-pass review workflow
- Slide 12 (5 min): Common failure patterns in practice
- Slide 13 (5 min): Activity
- classify and justify two ambiguous examples.
- Slide 14 (5 min): Debrief and bridge to segmentation/proofreading.
- Primary shortlist:
course/units/figures/07-glia-selected-v1.md.
- Include one class comparison table and one ambiguity panel.
Speaker notes (expert-level)
- Stress glia corrections as high-value QC, not optional cleanup.
- Tie glia labeling errors to false neuronal connectivity inferences.
Assessment and artifacts
- Deliverable: glia checklist with evidence requirements.
- Rubric dimensions: class discrimination quality and uncertainty handling.
Connections
Slide source file
- Marp draft source:
course/decks/marp/07-glia.marp.md
- Batch render helper:
./scripts/render_marp.sh
