Session profile
- Audience: annotators, proofreaders, and analysts needing ultrastructure fluency.
- Duration: 75 minutes lecture + 20 minutes image annotation drill.
- Output: labeled cue sheet with confidence scores and ambiguity notes.
Slide-by-slide lecture plan
- Slide 1 (2 min): Title and practical stakes
- Slide 2 (5 min): Ultrastructure as operational language
- Slide 3 (6 min): Compartment refresher
- Soma, dendrite, axon, boutons, spines.
- Slide 4 (6 min): Synapse architecture essentials
- Vesicles, active zone, PSD, cleft context.
- Slide 5 (6 min): Organelle cues
- Mitochondria, ER, microtubules in context.
- Slide 6 (6 min): Multi-slice evidence protocol
- Slide 7 (7 min): Worked example I
- Easy compartment call with converging evidence.
- Slide 8 (7 min): Worked example II
- Ambiguous case and confidence annotation.
- Slide 9 (6 min): Annotation disagreement analysis
- Slide 10 (5 min): Frequent error modes
- Slide 11 (5 min): QC metrics for ultrastructure calls
- Slide 12 (5 min): Standardized decision log template
- Slide 13 (7 min): Hands-on labeling prompt
- Slide 14 (7 min): Debrief and bridge to process classification.
- Primary shortlist:
course/units/figures/05-neuronal-ultrastructure-selected-v1.md.
- Use at least three cue-comparison panels and one ambiguity panel.
Speaker notes (expert-level)
- Require cue triangulation (morphology + organelle + context) before hard labels.
- Keep uncertain labels explicit; uncertainty is signal, not failure.
Assessment and artifacts
- Deliverable: cue-based annotation sheet with confidence tiers.
- Rubric dimensions: evidence quality, consistency, and uncertainty handling.
Connections
Slide source file
- Marp draft source:
course/decks/marp/05-neuronal-ultrastructure.marp.md
- Batch render helper:
./scripts/render_marp.sh
